10.05 Tailoring Small Molecules Using Glucosyltransferases (GLUTAIL)

Research

 

Plant secondary metabolites are an important source of active ingredients for food, cosmetic and pharmaceutical industry. They are frequently decorated with sugars resulting in both increased stability and water solubility of the glycoside product that also show unique targeting effects and altered activity when compared to the original scaffold. Glycosides are formed by the action of glycosyltransferases (GTs) which are a ubiquitous group of enzymes that catalyze the transfer of a sugar moiety from an activated sugar donor, usually UDP-glucose to acceptor molecules usually an alcohol. We are developing a process for the biotechnological production of small molecule glucosides with recombinant Escherichia coli. This is achieved by a joined effort of BINA (Biotechnology of Natural Products, WZW Freising) and IBE (Institute of Biochemical Engineering, Department of Mechanical Engineering, Garching).

We established and improved a scalable bioprocess for the production of whole-cell GT biocatalysts and designed the biocatalytic process step for the production of geranyl glucoside. The aroma glucoside geranyl glucoside can now be produced at L-scale in a stirred-tank reactor with subsequent purification and concentration of the product. However, the product concentrations after the whole-cell biotransformation step are still too low for industrial applications, and need to be further increased in future research efforts.

 

Publications

Katja Härtl, Kate McGraphery, Julian Rüdi-ger, Wilfried Schwab: "Tailoring Natural Products with Glycosyltransferases", 2017.

K. Härtl, F.-C. Huang, A. P. Giri, K. Franz-Oberdorf, J. Frotscher, Y. Shao, T. Hoffmann, W. Schwab: "Glucosylation of Smoke-Derived Vola-tiles in Grapevine (Vitis vinifera) is Catalyzed by a Promiscuous Resveratrol/Guaiacol Glucosyltransferase", 2017.

Priebe, XL., Daschner, M., Schwab, W., Weuster-Botz, D.: "Rational selection of biphasic reaction sys-tems for geranyl glucoside production by Escherichia coli whole-cell biocatalysts", 2017.

Mairinger T, Tröndle J, Hanscho M, Hann S.: "On-line clean-up and LC-MS analysis of primary metabolites in cell culture supernatants", 2017.

Gottardi M, Grün P, Bode HB, Hoffmann T, Schwab, W, Oreb M, Boles E.: "Optimisation of transcinnamic acid and hydrocinnamyl alcohol production with recombinant Saccharomyces cerevisiae and identification of cinnamyl methyl ketone as a by-product", 2017.

Weiner, M., Tröndle, J., Albermann, C. et al.: “Metabolic control analysis of L-phenylalanine production from glycerol with engineered E. coli using data from short-term steady-state perturbation experiments”, 2017.

Meo, A., Priebe, X., & Weuster-Botz, D. : "Lipid production with Trichosporon oleaginosus in a membrane bioreactor using microalgae hydrolysate", 2017.

Schmideder, A., Priebe, XL., Rubenbauer, M., Hoffmann, T., Huang, FC., Schwab, W., Weuster-Botz, D.: "Non-water miscible ionic liquid improves biocatalytic production of geranyl glucoside with Escherichia coli overexpressing a glucosyltransferase", 2016.

Weiner, M., Tröndle, J., Albermann, C., Sprenger, G., Weuster-Botz, D.: "Perturbation Experiments: Approaches for Metabolic Pathway Analysis in Bioreactors", 2016.

Valenzuela, R., Priebe, X., et al.: "Fiber modifications by organosolv catalyzed with H2SO4 improves the SSF of Pinus radiata", 2016.

Weiner, M., Tröndle, J., Schmideder, A., Binder, K., Albermann, C., Sprenger, G.A., Weuster-Botz, D.: "Parallelized small-scale production of uniformly 13C-labeled cell-extract for quantitative metabolome analysis", 2015.

Weiner, M., Tröndle, J., et. al.: "Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants”, 2014.

Albermann, C., Weiner, M., Tröndle J., Weuster-Botz, D., Sprenger, G. A.: "Utilization of organophosphate:phosphate antitransporter for isotope-labeling experiments in E. coli.”, 2014.

Weiner, M., Tröndle, J., Albermann, C., Sprenger, G. A., Weuster-Botz D.: "Carbon storage in recombinant Escherichia coli during growth on glycerol and lactic acid”, 2014.

Team

Project team leader

Dr. Thomas Hoffmann 
Biotechnology of Natural Products

Alumnus

Dr. rer. nat. Julian Rüdiger
Biotechnology of Natural Products

Alumna

Dr. rer. nat. Xenia Priebe
Chair of Biochemical Engineering

Alumna

Dr. rer. nat. Guangxin Sun
Biotechnology of Natural Products

Alumna

Dr.-Ing. Julia Tröndle
Chair of Biochemical Engineering

Principal investigator

Professor Wilfried Schwab
Head of Biotechnology of Natural Products

Principal investigator

Professor Dirk Weuster-Botz
Chair of Biochemical Engineering